source leaprc.protein.ff19SB
source leaprc.water.opc
prot = loadpdb 4LZT_amber.pdb
bond prot.6.SG prot.127.SG
bond prot.30.SG prot.115.SG
bond prot.64.SG prot.80.SG
bond prot.76.SG prot.94.SG
charge prot
check prot
saveamberparm prot 4LZT.prmtop 4LZT.inpcrd
quit